A case-control study on the polymorphisms of methylenetetrahydrofolate reductases, drinking interaction and susceptibility in colorectal cancer.
- Author:
Qin-ting JIANG
1
;
Kun CHEN
;
Xin-yuan MA
;
Xiao-ping MIAO
;
Kai-yan YAO
;
Wei-ping YU
;
Ling-yun LI
;
Yi-min ZHU
;
Hai-guang ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Alcohol Drinking; Case-Control Studies; China; Colorectal Neoplasms; enzymology; genetics; Female; Genetic Predisposition to Disease; genetics; Genotype; Humans; Male; Methylenetetrahydrofolate Reductase (NADPH2); genetics; Middle Aged; Polymerase Chain Reaction; Polymorphism, Genetic; Risk Factors
- From: Chinese Journal of Epidemiology 2004;25(7):612-616
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship between methylenetetrahydrofolate reductases (MTHFR) polymorphisms and colorectal cancer susceptibility.
METHODSA case-control study of 126 patients and 343 healthy controls was conducted to investigate the roles of MTHFR C677T and A1298C polymorphisms in colorectal cancer development. Genotypes of C677T and A1298C polymorphisms were analyzed by polymerase chain resction-restriction fragment length polymorphism (PCR-RFLP) methods.
RESULTSThe frequencies of MTHFR 677T and 1298C allele were 39.7% and 17.1%, respectively. After adjustment for age and sex, the MTHFR 1298C alleles seemed to have reduced association on the risk of colorectal cancer comparing to wild types. Among those with 677T and 1298A alleles, a decreased risk of colorectal cancer was observed: a 4-fold decrease in colorectal cancer risk (OR = 0.552, 95% CI: 0.265 - 1.150) in those with 677T and 1298C alleles. Men who were ex-drinkers and with MTHFR 1298C allele had a 2-fold increase in risk of colorectal cancer (OR = 3.307, 95% CI: 0.521 - 17.698) while no increased risk was seen among those current-drinkers.
CONCLUSIONSThis study suggested that certain MTHFR C677T and A1298C might be associated with the risk of colorectal cancer development. The interaction between MTHFR 1298AC polymorphisms and ex-drinking might also serve as a risk factor of colorectal cancer.
