Simultaneous determination of two saponnins in anemarrhenae rhizoma by HPLC-ELSD.
- Author:
Qianliang CHEN
1
;
Xiaoming SUN
;
Wenquan WANG
;
Changhua MA
Author Information
- Publication Type:Journal Article
- MeSH: Anemarrhena; chemistry; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; Saponins; analysis; Triterpenes
- From: China Journal of Chinese Materia Medica 2011;36(4):474-477
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish an HPLC-ELSD method for determination of Anemarsaponin C and Anemarsaponin A III in Anemarrhenae Rhizoma.
METHODKromasil C18 column(4.6 mm x 250 mm, 5 microm) was used as stationary phase. Mobile phase was methanol-water gradient with the flow rate of 1 mL x min(-1); the temperature of the drift tube and evaporation was 50 degrees C and 70 degrees C respectively. The gas pressure was 1.03 x 10(5) Pa.
RESULTThere are good linearity in the range 0.310-3.10 microg of anemarsaponin C (lgA = 1.254 2lgM + 5.734 7, r = 0.999 5) and in the range 0.323-3.23 microg (lgA = 1.328 41gM + 5. 937, r = 0.999 6) of anemarsaponin A III. The average recovery of anemarsaponin C and anemarsaponin A III was 98.1% with RSD 2.1% and 97.3% with RSD 1.5% (n = 6) respectively.
CONCLUSIONThe method is rapid and accurate. It is suitable for quality control of Anemarrhenae Rhizoma. The result of determination reveals that the quality of Anemarrhenae Rhizoma from different places of north China are of notable difference.
