Optimization of a HPLC determination method for Evodia rutaecarpa.
- Author:
Zhifang HUANG
1
;
Jinhai YI
;
Yan WU
;
Yunhua LIU
;
Yan CHEN
;
Yuhong LIU
Author Information
1. Sichuan Academy of Chinese Medicine Sciences, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid;
methods;
Evodia;
chemistry;
Indole Alkaloids;
analysis;
Limonins;
analysis;
Plant Extracts;
analysis;
Quinazolines;
analysis
- From:
China Journal of Chinese Materia Medica
2011;36(4):478-480
- CountryChina
- Language:Chinese
-
Abstract:
A HPLC method for determination of limonin, evodiamine and rutaecarpine in Evodia rutaecarpa was optimized. The mobile phase was [acetonitrile-tetrahydrofuran (25: 15)] -0.02% H3 PO4 (35:65). The detection wavelength was 220 nm and the flow rate was 1.0 mL x min(-1). Limonin, evodiamine and rutaecarpine were all well separated from other substances and their UV spectrums were essentially the same to the standards . The liner ranges of limonin, evodiamine and rutaecarpine were 0.196 8-3.936, 0.153 6-3.072, 0.097 4-1.948 microg. The average recoveries were 97.8%, 100.7% and 98.4%. RSD were 1.7%, 1.3% and 1.1% (n = 6). The method of this article is accurate, reproducible and can be used to enhance the quality control of E. rutaecarpa.
