HPLC determination of the contents chlorogenic acid and hydrochlorothiazide in zhenjujiangyapian.
- Author:
Qinhong GONG
1
;
Jian RUAN
Author Information
- Publication Type:Journal Article
- MeSH: Antihypertensive Agents; analysis; Chlorogenic Acid; analysis; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; Hydrochlorothiazide; analysis
- From: China Journal of Chinese Materia Medica 2011;36(4):481-483
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish an HPLC method for determination of chlorogenic acid and hydrochlorothiazide in Zhenjujiangyapian.
METHODThe HPLC method was carried on C18 column using methanol-0.1% phosphoric acid(20: 80) as mobile phase, and the detection wavelength was 327 nm, the flow rate was 1.0 mL x min(-1) and the temperature of column was 40 degrees C.
RESULTIn the HPLC method, the calibration curve for chlorogenic acid, hydrochlorothiazide were linear in the range of 0.049 6-0.496 (r = 0.999 5) and 1.002-10.02 microg (r = 0.999 8). The average recovery for chlorogenic acid, hydrochlorothiazide were 101.0% and 100. 1%. RSD were 2.0% and 1.4% (n = 9), respectively.
CONCLUSIONThe method is convenient, precise and reliable for determining the content of chlorogenic acid and hydrochlorothiazide in Zhenjujiangyapian.
