Inhibition of three pentacyclic triterpenoids on calcium-induced liver mitochondrial permeability transition in mice.

Xinhui Tang; Lifang Yan; Lizhi XU; Jing Gao

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Inhibition of three pentacyclic triterpenoids on calcium-induced liver mitochondrial permeability transition in mice.

Author: Xinhui TANG ¹ ; Lifang YAN ; Lizhi XU ; Jing GAO
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1. Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Life Science and Technology, Yancheng Teachers College, Yancheng 224051, China.
Publication Type:Journal Article
MeSH: Animals; Calcium; metabolism; pharmacology; Membrane Potential, Mitochondrial; drug effects; Mice; Mice, Inbred ICR; Mitochondria, Liver; drug effects; metabolism; Mitochondrial Membrane Transport Proteins; drug effects; Mitochondrial Swelling; drug effects; Pentacyclic Triterpenes; pharmacology
From: China Journal of Chinese Materia Medica 2011;36(4):496-499
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo study effects of three pentacyclic triterpenoids, oleanolic acid (OA), ursolic acid (UA) and asiatic acid (AA) on Ca(2+)-induced liver mitochondrial permeability transition (MPT).
METHODEffects of three compounds on liver MPT induced by Ca2+ were assessed by measuring the change in mitochondrial swelling, mitochondrial membrane potential and release of matrix Ca2+ in vitro.
RESULTObvious mitochondrial swelling, loss of mitochondrial membrane potential and release of matrix Ca2+ occurred after the addition of 50 micromol x L(-1) Ca2+. However, preincubation with 50 mg x L(-1) OA, UA or AA significantly blocked the above changes. In addition, it was also found that there are differences in the inhibitions of three compounds on liver MPT induced by Ca2+.
CONCLUSIONThree pentacyclic triterpenoids, OA, UA and AA, have significant mitochondrial protection through blocking on liver MPT and the inhibition on liver MPT of AA is stronger than that of UA and OA.