Molecular identification of one Uncaria plant.
- Author:
Shuang ZHU
1
;
Lin ZHOU
;
Huimin PANG
;
Hongliang HUANG
;
Xiaoxia GAO
;
Changqing ZENG
Author Information
- Publication Type:Journal Article
- MeSH: DNA, Plant; genetics; DNA, Ribosomal Spacer; genetics; Phylogeny; Sequence Homology, Nucleic Acid; Species Specificity; Uncaria; classification; genetics
- From: China Journal of Chinese Materia Medica 2011;36(5):535-537
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEIn order to identify a species of Uncaria, molecular phylogenetic analysis was carried out by using the rDNA ITS sequence as molecular marker.
METHODTotal DNA was extracted from the plant with modified CTAB method and thereby rDNA ITS regions were amplified with universally conserved primer. The rDNA ITS amplicon was characterized by cloning, sequencing, blasting in GenBank and phylogenetic analyses using PAUP by maximum parsimony (MP) criteria.
RESULTThe rDNA ITS entire sequence of this species of Uncaria was 719 bp. The sequence is related to the U. sinensis available in GenBank and the similarity reaches 99.7%.
CONCLUSIONBased on molecular biology methods of rDNA ITS region analysis, molecular identification is available in accurate classification on this species of Uncaria.
