Optimization of the hydrolysis process for C21 steroidal glycoside of bai shou wu by acetic acid with multi-target orthogonal design.
- Author:
Xin ZHAO
1
;
Mei CHEN
;
Yunru PENG
;
Youbin LI
Author Information
- Publication Type:Journal Article
- MeSH: Acetates; pharmacology; Cynanchum; chemistry; metabolism; Drugs, Chinese Herbal; chemistry; metabolism; Glycosides; analysis; chemistry; metabolism; Hydrolysis; drug effects; Temperature; Time Factors
- From: China Journal of Chinese Materia Medica 2011;36(5):569-572
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the optimizal hydrolysis process for C21 steroidal glycoside of Bai Shou Wu by acetic acid.
METHODThe effects of acetic acid concentration, reaction temperature and reaction time had been investigated using orthogonal design and the contents of kidjoranin 3-O-beta-digitoxopyranoside, caudatin, kidjoranin 3-O-alpha-L-diginopyranosyl-(1 --> 4)-beta-cymaropyranoside and caudatin 3-O-beta-cymaropyranoside as response indexs were determined by the high performance liquid chromatography.
RESULTThe factors influencing acetic extraction efficiency were as follows: A > B > C (A. reaction temperature; B. reaction time; C. acetic acid concentration). The optimal hydrolysis condition obtained was: refluxing for 6 hours with 5.0% dilute CH3COOH solution at 100 degrees C.
CONCLUSIONThe content of antitumor active ingredients under the optimum hydrolysis condition is raised obviously and has a great part in studying this antitumor drug.
