Simultaneous determination of seven nucleosides and nucleobases in aqueous extracts of Fritillaria taipaiensis by HPLC-DAD.
- Author:
Linfang HUANG
1
;
Baozhong DUAN
;
Lizhi WANG
;
Dahua WEI
;
Qifu LU
;
Shilin CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; chemistry; Fritillaria; chemistry; Nucleosides; analysis; Reproducibility of Results; Water; chemistry
- From: China Journal of Chinese Materia Medica 2011;36(5):585-588
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a high performance liquid chromatography coupled with a photodiode array detector (HPLC-DAD) method for simultaneous determination of 7 nucleosides and nucleobases in Fritillaria taipaiensis.
METHODThe analyses were performed on an Agilent Zorbax-SB-Aq-C18 column (4.6 mm x 250 mm, 5 microm) eluted with water and methanol in gradient mode. The flow rate was 1.0 mL x min(-1). The detection wavelength was 260 nm. The temperature of sample manager was set at 25 degrees C, and the injection volume was 20 microL.
RESULTThe investigated compounds including uracil, cytidine, uridine, guanosine, thymidine, adenosine and adenine were shown good linearity (r > or = 0.999 8) over the tested ranges. The average recoveries were within 96.96% - 103.5% with RSD < or = 3.8%.
CONCLUSIONThe accuracy, stability, repeatability and average recovery of the method are satisfying, and the seven nucleosides and nucleobases components in F. taipaiensis can be rapidly and accurately quantified by HPLC-DAD. This work provided helpful information for comprehensive quality evaluation of F. taipaiensis.
