Determination of mangiferin in rat plasma and aqueous humor by HPLC.
- Author:
Shengjun FAN
1
;
Yuanqin GU
;
Guoyu LI
;
Mingyu ZHANG
;
Baoxin LI
;
Hong ZHANG
;
Yunlong HOU
Author Information
1. Department of Pharmacology, Harbin Medical University, Harbin 150086, China. herryvan@sohu.com
- Publication Type:Journal Article
- MeSH:
Animals;
Aqueous Humor;
chemistry;
Chromatography, High Pressure Liquid;
Female;
Male;
Nitrophenols;
analysis;
Rats;
Rats, Wistar;
Reproducibility of Results;
Sensitivity and Specificity;
Xanthones;
blood;
pharmacokinetics
- From:
China Journal of Chinese Materia Medica
2011;36(5):598-602
- CountryChina
- Language:Chinese
-
Abstract:
A HPLC method was developed for the determination of mangiferin in rat plasma and aqueous humor. 4-Nitrophenol was used as internal standard. Analysis was performed on a Cosmosil ODS C18 analytical column (4.6 mm x 250 mm, 5 microm) with mobile phase consisting of methanol-water (40: 60) with 2% glacial acetic acid at a flow rate of 1.0 mL x min(-1). The calibration curve of mangiferin in rat plasma and aqueous humor showed excellent linear behaviors over the investigated concentration of 0. 50-250.00 mg x L(-1) in plasma and 0.10-10.00 mg x L(-1) in aqueous humor, respectively, and the correlation coefficients were all above 0.995 4. The intra-day and inter-day precisions for all samples were measured to be below 12%. The limit of quantitation was 0.10 mg x L(-1) and low enough for the determination of mangiferin in all samples. The validated method has been successfully applied to preliminary pharmacokinetics study of mangiferin in rat plasma and aqueous humor.