Rapid determination of puerarin in Xiaoke pill powder by near-infrared spectroscopy.
- Author:
Biru SU
1
;
Bin YE
;
Chunxian GENG
;
Meng SHI
;
Changhai WU
Author Information
- Publication Type:Journal Article
- MeSH: Drugs, Chinese Herbal; analysis; Isoflavones; analysis; Powders; Spectroscopy, Near-Infrared; methods; Time Factors
- From: China Journal of Chinese Materia Medica 2011;36(6):672-675
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the content of puerarin in Xiaoke pill powder rapidly by near-infrared spectroscopy.
METHODNear-infrared diffuse reflectance spectroscopy technology was used to collect NIR spectra of Xiaoke pills powder. With HPLC analysis values as reference, the fast determination method of puerarin was established with partial least squares (PLS).
RESULTThe R2, RMSECV and RPD of the calibration model for puerarin were 0.980 1, 0.131 and 7.09. The average relative deviation of the predication set was 3.2%.
CONCLUSIONThe method is accurate, fast and simple, which could be generalized to the on-line quality control of Xiaoke pill powder production.
