Metabolomics study of anti-inflammatory action of Radix Paeoniae Rubra and Radix Paeoniae Alba by ultraperformance liquid chromatography-mass spectrometry.
- Author:
Qiwei YANG
1
;
Li YANG
;
Aizhen XIONG
;
Lan LU
;
Rui WANG
;
Zhengtao WANG
Author Information
- Publication Type:Journal Article
- MeSH: Alprostadil; analogs & derivatives; blood; Animals; Anti-Inflammatory Agents; pharmacology; Chromatography, Liquid; methods; Dinoprost; blood; Male; Metabolomics; Paeonia; Plant Extracts; pharmacology; Rats; Rats, Sprague-Dawley; Spectrometry, Mass, Electrospray Ionization; methods
- From: China Journal of Chinese Materia Medica 2011;36(6):694-697
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the anti-inflammatory effect of Radix Paeoniae Rubra and Radix Paeoniae Alba by animal experiment and metabolimic analysis.
METHODTo establish the rats model of toes swelling caused by carrageenan, study the anti-inflammatory effect of Radix Paeoniae Rubra and Radix Paeoniae Alba. The serum samples were analyzed by ultraperformance liquid chromatography-mass spectrometry (UPLC-MS), to find out the potential identification biomarker by PLS-DA.
RESULTBoth of the extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba have good effects of inhibition to swelling caused by carrageenan in 0.5-1 h, and the extract of Radix Paeoniae Rubra also show significant inhibition in 2-3 h. Glutathione( GSH), gamma-aminobutyric acid (GABA), prostaglandin F2alpha (PGF2alpha), prostaglandinE3 (PGE3), leukotrieneA4 (LTA4), prostaglandinE2 ( PGE2) are proven to be significant expressed biomarkers. Radix Paeoniae Rubra and Radix Paeoniae Alba may have great influence on PGF2alpha and PGE3. There was also significant difference between the effects of Radix Paeoniae Rubra and Radix Paeoniae Alba, which suggested the difference of anti-inflammatory between the two herbs.
CONCLUSIONThe results of metabolomics are related with the results of classic pharmaco- experiment, which is helpful for the further research of the mechanism of action of Radix Paeoniae Rubra and Radix Paeoniae Alba.
