Metabolomics study of anti-platelet effect of Radix Paeoniae Rubra and Radix Paeoniae Alba by UPLC-MS.
- Author:
Qiwei YANG
1
;
Li YANG
;
Aizhen XIONG
;
Rui WANG
;
Zhengtao WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Chromatography, Liquid; methods; Least-Squares Analysis; Male; Mass Spectrometry; methods; Metabolomics; Paeonia; Plant Extracts; pharmacology; Platelet Aggregation Inhibitors; pharmacology; Rats; Rats, Sprague-Dawley
- From: China Journal of Chinese Materia Medica 2011;36(6):698-701
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the anti-platelet effect and influence of Radix Paeoniae Rubra and Radix Paeoniae Alba on rat's endogenous metabolites by animal experiment and UPLC-MS based metabolomic method.
METHODAfter administration of 80% ethanol extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba for 6 d, the serum samples were analyzed by ultra-performance liquid chromatography- mass spectrometry (UPLC-MS) and partial least squares-discriminant analysis (PLS-DA) to find out the potential biomarker.
RESULTBoth of the extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba have good effects of inhibition on platelet coacervation, and the effect of Radix Paeoniae Alba is better than that of Radix Paeoniae Rubra. Malic acid, alpha-acetone dicarboxylic acid, leukotrieneA4 (LTA4), prostaglandinE2 (PGE2) and prostaglandin F2alpha (PGF2alpha) are proved to be significant expressed biomarkers.
CONCLUSIONMetabolomics is helpful for the further research of the mechanism of anti-platelet action of Radix Paeoniae Rubra and Radix Paeoniae Alba.
