Simultaneous determination of five constituents in Scrophularia ningpoensis by HPLC.
- Author:
Xuemei ZHANG
1
;
Rui WANG
;
Rui AN
;
Ximin WU
;
Xinhong WANG
;
Yiming LI
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Cinnamates; analysis; Coumaric Acids; analysis; Glucosides; analysis; Glycosides; analysis; Iridoid Glycosides; Phenols; analysis; Pyrans; analysis; Scrophularia; chemistry; Trisaccharides; analysis
- From: China Journal of Chinese Materia Medica 2011;36(6):709-711
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for the simultaneous quantitation of five constituents in Scrophularia ningpoensis.
METHODSamples were analyzed on an Agilent SB-C18 column(4.6 mm x 250 mm, 5 microm) eluted with acetonitrile and water containing 0.03% phosphate acid as mobile phases in a linear gradient mode. The flow rate was kept at 1.0 mL x min(-1), and the column temperature was set to 30 degrees C. The DAD detector wavelengths were 210, 280, 330 nm.
RESULTThe linear ranges were 50-400 mg x L(-1) for harpagide, 1-40 mg x L(-1) for harpagoside, 1-20 mg x L(-1) for cinnamic acid, 0.5-4.5 mg x L(-1) for acteoside,1-60 mg x L(-1) for angoroside C, respectively. The average recoveries of the five constituents were 100.8% (RSD 0.62%), 101.7% (RSD 0.32%), 98.8% (RSD 0.48%), 99.9% (RSD 1.4%), 99.2% (RSD 1.1%), respectively.
CONCLUSIONThrough the validation, the method was proved to be sensitive, accurate, repeatable, and can be used for quality control of the roots of S. ningpoensis.
