Characteristic chromatographic profile of Paeoniae Radix and its application in quality control of crude and processed drugs of different origins and processing methods.
- Author:
Rui WANG
1
;
Shanjun HUANG
;
Qiwei YANG
;
Yanhong SHI
;
Zhengtao WANG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Paeonia; chemistry; Quality Control
- From: China Journal of Chinese Materia Medica 2011;36(6):729-733
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a HPLC method of characteristic chromatographic profile for the quality control of Paeoniae Radix.
METHODThe 67 batches of samples were analyzed on a Polaris C18-A column with a gradient elution of acetonitrile and phosphate solution (pH 3.0) at a flow rate of 1.0 mL x min(-1) and detected at 230 nm.
RESULTNine main marker peaks were identified and semi-quantificated. By the similarity evaluation software for chromatographic fingerprint of traditional Chinese medicine (Version 2004A) and hierarchical clustering analysis, 67 batches of Paeoniae Radix were classified.
CONCLUSIONThe method can be applied for quality assessment of Paeoniae Radix.
