Isolation of endophytic fungi from Huperzia serrata and their acetylcholinesterase inhibitory activity.
- Author:
Ya WANG
1
;
Qinggui ZENG
;
Zhibin ZHANG
;
Riming YAN
;
Lingyun WANG
;
Zhu DU
Author Information
1. Key Laboratory for Research on Active Ingredients in Natural Medicine of Jiangxi Province, Yichun University, Yichun 336000, China.
- Publication Type:Journal Article
- MeSH:
Cholinesterase Inhibitors;
pharmacology;
Fungi;
classification;
isolation & purification;
Huperzia;
microbiology
- From:
China Journal of Chinese Materia Medica
2011;36(6):734-740
- CountryChina
- Language:Chinese
-
Abstract:
A total of 127 strains of endophytic fungi were isolated from roots, branches and leaves of Huperzia serrata. These strains were identified into 19 genera based on morphological characters and ribosomal DNA (rDNA) sequence analysis, there into Penicillium, Aspergillus and Podospora were dominant populations in H. serrata. From analysis results we found some endophytic fungi showed a certain degree of tissue preference. The isolation rate and colonization rate of stems were both larger than those of leaf and roots. After testing the acetylcholinesterase (AChE) inhibitory activity of these endophytic fungi, a total of 39 endophytic fungi belonging to 15 genera showed AChE inhibition. Eleven endophytic fungi showed potent AChE inhibition, 7 of which were isolated from leaf. The research not only provided theoretical basis for developing and utilizing the resources of endophytic fungi in H. serrata but also showed a new path for searching medicines resource which has AChE inhibitory activity.
