Simultaneous characterization of two types of major active components in Kushen by high performance liquid chromatography coupled to multi-stage mass spectrometry.
- Author:
Qinqin ZHAO
1
;
Yufeng ZHANG
;
Xiaohui FAN
Author Information
1. Department of Chinese Medicine Science & Engineering, Zhejiang University, Hangzhou 310058, China.
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid;
methods;
Drugs, Chinese Herbal;
chemistry;
Spectrometry, Mass, Electrospray Ionization;
methods
- From:
China Journal of Chinese Materia Medica
2011;36(6):762-769
- CountryChina
- Language:Chinese
-
Abstract:
In this study, a high performance liquid chromatography coupled with multi-stage mass spectrometry method was developed for simultaneous characterization of alkaloids and flavonoids, the main active components, in Kushen with diverse physical and chemical properties. Forty-two major constituents, including sixteen Kushen alkaloids and twenty-six Kushen flavonoids were tentatively identified. Additionally, useful and characteristic fragmentation pathways of two types of Kushen alkaloids, namely cytisine-type and sparteine-type, in positive ions mode were proposed and summarized, which would lay a foundation for the rapid identification of the active ingredients in traditional Chinese medicine Kushen.
