Simultaneous assay of paeoniflorin and albiflorin in Paeoniae Radix Alba by QAMS.
- Author:
Shanjun HUANG
1
;
Qiwei YANG
;
Yanhong SHI
;
Rui WANG
;
Zhengtao WANG
Author Information
- Publication Type:Journal Article
- MeSH: Benzoates; analysis; Bridged-Ring Compounds; analysis; Chromatography, High Pressure Liquid; methods; Glucosides; analysis; Monoterpenes; Paeonia; chemistry
- From: China Journal of Chinese Materia Medica 2011;36(6):780-783
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a method of quantitative analysis of multi-components by single marker (QAMS) for simultaneously determining paeoniflorin and albiflorin in Paeoniae Radix Alba.
METHODUsing paeoniflorin as the internal reference substance, the relative correction factor (RCF) of albiflorin was determined by HPLC and UPLC with good reproducibility. The contents of paeoniflorin in 16 samples of Paeoniae Radix Alba were authentically determined by the external standard method, and the content of albiflorin was calculated according to the RCF. The contents of these two components in the samples were determined with the external standard method.
RESULTNo siginificant differences between the quantitative results of QAMS method and external standard method were observe.
CONCLUSIONIt is a convenient and accurate method to determine multi-components when some authentic standard substances were unavailable. It can be used to control the quality of Paeoniae Radix Alba
