Research methods of anti-HIV-1 inhibitors targeting at Vif-APOBEC3G axis.
- Author:
Xinhua QIAO
1
;
Wenjun ZHANG
;
Zelin LI
;
Yi ZENG
Author Information
1. College of Life Science, Beijing University of Technology, Beijing 100124, China.
- Publication Type:Journal Article
- MeSH:
APOBEC-3G Deaminase;
Anti-HIV Agents;
pharmacology;
Blotting, Western;
Cytidine Deaminase;
antagonists & inhibitors;
Fluorescence;
HIV-1;
drug effects;
Immunoprecipitation;
Surface Plasmon Resonance;
vif Gene Products, Human Immunodeficiency Virus;
antagonists & inhibitors
- From:
China Journal of Chinese Materia Medica
2011;36(6):806-809
- CountryChina
- Language:Chinese
-
Abstract:
The mammalian APOBEC3G protein (apolipoprotein B mRNA-editing enzyme catalytic polypeptide 3 protein G, APOBEC3G) is an important component of the cellular innate immune response to retroviral infection. APOBEC3G can extinguish HIV-1 (human immunodeficiency virus type 1) infectivity by its incorporation into virus particles and subsequent cytosine deaminase activity to block replication of HIV-1. HIV-1 Vif (viral infectivity factor) suppresses various APOBEC3 proteins through a common mechanism which induces the degradation of target proteins. Therefore, the interrelation of Vif-APOBEC3G has been extensively studied, which represents attractive targets for the development of novel inhibitors. We summarize the papers in which the detection technique and methods have been developed to assay the anti-HIV activity and its mechanism, such as western-blotting, co-immunoprecipitation, pulse-chase experiments, bioluminescence resonance energy transfer, biomolecular interaction analysis. This review is towards developing therapeutics aimed at the Vif-APOBEC3G axis.
