Influence of different processing methods on Angelica sinensis polysaccharides from same origin.
- Author:
Jieli LV
1
;
Hongli CHEN
;
Jinao DUAN
;
Hui YAN
;
Yuping TANG
;
Bingsheng SONG
Author Information
- Publication Type:Journal Article
- MeSH: Angelica sinensis; chemistry; Animals; Cell Proliferation; drug effects; Drug Compounding; methods; Drugs, Chinese Herbal; chemistry; Mice; Polysaccharides; chemistry; isolation & purification; pharmacology; Spleen; cytology; T-Lymphocytes; cytology; drug effects
- From: China Journal of Chinese Materia Medica 2011;36(7):846-849
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the influences of different processing methods on the content of Angelica sinensis polysaccharides (APS) from the same origin.
METHODThe contents of neutral polysaccharides and acidic polysaccharides in various samples of A. sinensis were determined by phenol-sulfuric acid and carbazole-sulfuric acid method, respectively. The proliferation ability of lymphocyte was detected by MTT method after the cells were cultured with different concentrations of APS from two samples processed by different methods.
RESULTThe different processing methods had different effects on the contents of polysaccharide. The maximum content of APS (26.03%) was found in the sample processed by microwave drying medium-fired, but the minimum content of APS (2.25%) was found in the sample processed by vacuum drying at 50 TC. Furthermore, the APS (high concentration group, P < 0.01) processed by microwave drying medium-fired could both accelerate proliferation of spleen lymphocytes directly and increase proliferation of T cells of mice induced by Con A. However, the APS processed by far-infrared drying did not show conspicuous immune enhancement activity.
CONCLUSIONDifferent processing methods have different effects on the contents of APS and the proliferation ability of lymphocytes.
