Determination of evodiamine and rutacarpine in zhuyu hewei zhitong capsules by HPLC.
- Author:
Xiuping MA
1
;
Jiang LI
;
Yingbo LIU
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; analysis; Capsules; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; chemistry; standards; Linear Models; Quality Control; Quinazolines; analysis; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2011;36(7):871-873
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a HPLC method for the determination of evodiamine and rutacarpine in Zhuyu Hewei Zhitong capsules.
METHODThe separation was performed at 30 degrees C on an Eclipse XDB-C18 column (4.6 mm x 150 mm, 5 microm) eluted with the mobile phase consisted of acetonitrile-water(45:55). The detection wavelength was 250 nm.
RESULTSThe standard curve for evodiamine is linear in the range of 0.0510-0.560 microg, the average recovery was 97.5% (RSD 1.0%). The standard curve for rutacarpine is linear in the range of 0.0508-0.559 microg, the average recovery was 98.7% (RSD 1.5%).
CONCLUSIONThe method is specific, accurate and stable. It can be used for detemination of evodiamine and rutacarpine in Zhuyu Hewei Zhitong capsules.
