Non-invasive prenatal genetic diagnosis using multiple displacement amplification.
- Author:
Wei-yu LIU
1
;
Chun-lian JIN
;
Li-ying LIU
;
Chang-kun LIN
;
Yan WANG
;
Kai-lai SUN
Author Information
- Publication Type:Journal Article
- MeSH: Erythroblasts; metabolism; Feasibility Studies; Female; Fetal Diseases; blood; diagnosis; genetics; Humans; Muscular Dystrophy, Duchenne; blood; diagnosis; genetics; Polymerase Chain Reaction; methods; Pregnancy; Prenatal Diagnosis; methods
- From: Chinese Journal of Medical Genetics 2007;24(2):196-199
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the feasibility of multiple displacement amplification (MDA) to apply in the non-invasive prenatal genetic diagnosis of Duchenne muscular dystrophy (DMD).
METHODSMaternal blood was obtained from 20 pregnant women at 7 to 25 weeks of gestation. After the discontinuous density gradient centrifugation with Percoll, the fetal nucleated red blood cells (NRBCs) were stained with Kleihauer test. All positive NRBCs were collected by micromanipulator and then performed with MDA. Sex and short tandern repeat (STR) analysis were determind from a small aliquot of the reaction. The origin of NRBCs was verified and prenatal diagnosis of DMD was made at the same time.
RESULTSThe product length of MDA was >15 kb, while primer extension preamplification (PEP) is only about 1 kb. We completed non-invasive prenatal genetic diagnosis of 6 fetus at high risk of DMD using MDA. The results were all coincident with amniotic fluid control.
CONCLUSIONThe MDA method which provides a highly uniform representation across the genome, representing the entire genome with minimal amplification bias, shows good application prospects.
