Application of real-time fluorescence quantitative PCR accompanied with comparison of Delta CT for diagnosis of Down's syndrome from a single cell.
- Author:
Rong YU
1
;
Han-ping CHEN
;
Xiao-fang YAN
Author Information
- Publication Type:Journal Article
- MeSH: Child, Preschool; Down Syndrome; blood; diagnosis; genetics; Female; Fetal Blood; cytology; metabolism; Humans; Infant; Lymphocytes; cytology; metabolism; Male; Microsatellite Repeats; genetics; Polymerase Chain Reaction; methods; Pregnancy; Prenatal Diagnosis; methods; Reproducibility of Results; Sensitivity and Specificity
- From: Chinese Journal of Medical Genetics 2007;24(2):200-202
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the use of real-time fluorescence quantitative PCR (FQ-PCR) accompanied with comparison of Delta CT as a method for diagnosis of Down's syndrome from a single cell.
METHODSSingle lymphocyte was isolated from the peripheral or umbilical cord blood samples of 22 clinically diagnosed Down's syndrome patients or fetus and 40 normal controls by micromanipulation techniques. Primer extension preamplification (PEP) and real-time FQ-PCR were used to amplify the S100B and DCSR1 located on chromosome 21, and GAPDH located on chromosome 12. Two pairs of DeltaCT values were compared between the two groups. The ratios of S100B/GAPDH and DSCR1/GAPDH products were calculated in trisomy 21 group.
RESULTSThe DeltaCT values of Down's syndrome patients were significantly lower than those of normal controls. The difference between the two groups was statistically significant (P<0.01). The ratios of S100B/GAPDH and DSCR1/GAPDH products for trisomy 21 were 1.891(1.563-2.287) and 1.840 (1.562-2.168), respectively.
CONCLUSIONReal-time FQ-PCR is a reliabe method that may provide a new way for non-invasive prenatal diagnosis and preimplantation genetic diagnosis for Down's syndrome.
