Meiotic segregation results of male reciprocal chromosome translocations.
- Author:
Xue-feng HUANG
1
;
Shi-quan XIAO
;
Qian-jin FEI
;
Wei ZHANG
;
Li-ya ZHANG
;
Xu YANG
;
Bi-lv YE
Author Information
- Publication Type:Journal Article
- MeSH: Chromosome Breakage; Heterozygote; Humans; In Situ Hybridization, Fluorescence; Karyotyping; Male; Meiosis; genetics; Spermatozoa; metabolism; Translocation, Genetic; genetics
- From: Chinese Journal of Medical Genetics 2007;24(2):217-220
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the meiotic segregation results of male reciprocal chromosome translocation by fluorescence in situ hybridization (FISH).
METHODSMulti-color FISH using 3 combined probes located in any 3 chromosome segments on both sides of two breakpoints was performed on the de-condensed sperm head to analyze the sperm chromosomal contents and segregation patterns.
RESULTSFour male reciprocal translocation carriers were included in the study, with the karyotypes of 46, XY, t(2;18) (p16; q23); 46, XY, t(4;6) (q34;q21); 46, XY, t(8;13) (q23;q21) and 46, XY, t(4;5) (4q31;5q13), respectively. The results showed that 4 carriers had different proportions of various segregated spermatozoa. The spermatozoa of alternate, adjacent-1, adjacent-2, 3:1, non-disjunction in meiosis II, and 4:0 or diploidy accounted for 27.1%-49.4%, 26.9%-37.6%, 2.7%-15.7%, 8.6%-32.7%, 0.2%-1.9%, and 0.1%-0.4%, respectively.
CONCLUSIONFor each-reciprocal translocation carrier seems to have a particular meiotic segregation results, FISH analysis on sperm head should be done for each carrier in order to provide an accurate genetic counseling.
