Overexpression of resistin affect 3T3-L1 adipocyte lipid metabolism.
- Author:
Nan GU
1
;
Xi-rong GUO
;
Yu-hui NI
;
Feng LIU
;
Li FEI
;
Rong-hua CHEN
Author Information
- Publication Type:Journal Article
- MeSH: 3T3-L1 Cells; Adipocytes; metabolism; Animals; Cell Differentiation; genetics; Fatty Acids, Nonesterified; metabolism; Gene Expression; Glucose Transporter Type 4; genetics; Lipid Metabolism; genetics; Mice; Rats; Resistin; genetics; metabolism; Triglycerides; metabolism
- From: Chinese Journal of Medical Genetics 2007;24(3):251-255
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of resistin overexpression on 3T3-L1 adipocyte lipid and glucose metabolism.
METHODSExpression vector for rat resistin gene was constructed and transfected into 3T3-L1 adipocytes. Cell differentiation and lipid accumulation was determined by Oil Red O staining. Differentiation marker genes (pref-1, C/EBPalpha, FAS) and glucose transporter 4 (GLUT4) gene mRNA expressions were evaluated by reverse transcription-PCR (RT-PCR). Triglyceride (TG) and free fatty acids (FFAs) in adipocytes were measured by colorimetric kit.
RESULTS(1) In resistin-overexpressed adipocytes, the lipid droplets presented at the second day which was earlier than the control cells. (2) The expression of C/EBPalpha and FAS genes in resistin-overexpressed adipocytes were up-regulated and the pref-1 was down-regulated compared with that of the control cells. (3) In resistin-overexpressed adipocytes, cellular TG and FFAs levels were significantly increased (P<0.05). (4) There was no difference in the expression of GLUT4 gene between 3T3-L1 adipocytes and resistin-overexpressed adipocytes (P> 0.05).
CONCLUSIONOverexpression of resistin can affect 3T3-L1 adipocyte lipid metabolism and thereby result in obesity and insulin resistance, but have no effect on GLUT4 gene expression.
