Influence of interphase nuclei preparation techniques on fluorescence in situ hybridization results in solid tumors.
- Author:
Ya-ling HAN
1
;
Xin XU
;
Yan CAI
;
Ming-rong WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Fractionation; methods; Cell Nucleus; metabolism; Collagenases; metabolism; In Situ Hybridization, Fluorescence; methods; Interphase; Neoplasms; genetics; pathology
- From: Chinese Journal of Medical Genetics 2007;24(3):331-333
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a technology platform for the preparation of interphase nuclei for the fluorescence in situ hybridization (FISH) detection of solid tumor tissues.
METHODSThe centromere probe of chromosome 3 was labeled by the random primer technique, and then hybridized to interphase nuclei prepared by six different methods in order to study the influence on FISH detection.
RESULTSEach method of slide preparation had its own characteristic, and could be used according to different needs. As regards to FISH, collagenase method got the best results. Whereas for frozen samples or small tissues, to prepare printing slides was more applicable.
CONCLUSIONThe comparison of different slide preparation methods lays a technology foundation for the FISH application in cancer researches and clinical diagnosis of solid tumors.
