Rapid detection of chromosomes X and Y using dual-color primed in situ labeling technique.

Author: Jian-bin YANG ¹ ; Zheng-yan ZHAO
Author Information

1. Children's Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310003 P. R. China.
Publication Type:Journal Article
MeSH: Adolescent; Adult; Chromosome Disorders; diagnosis; genetics; Chromosomes, Human, X; genetics; Chromosomes, Human, Y; genetics; Female; Humans; Pregnancy; Primed In Situ Labeling; methods; Reproducibility of Results; Sensitivity and Specificity; Young Adult
From: Chinese Journal of Medical Genetics 2007;24(4):477-478
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo develop a rapid method for detection of chromosomes, and to present a feasible method for rapid detection of the interphase nuclei of uncultured amniocytes using modified dual-color primed in situ labeling (PRINS) technique.
METHODSChromosomes X and Y were detected and analyzed by the modified dual-color PRINS in 205 amniotic fluid samples.
RESULTSThe specific chromosomes were obtained on both metaphase and interphase nuclei. In more than 98% of the samples PRINS reactions with primers X and Y were successfully induced. One sample was properly identified and correctly scored as 47, XXY.
CONCLUSIONThe results suggest that PRINS technique is rapid, simple and cost-effective. It is also sensitive and specific, and thus useful for rapid detection of chromosome abnormalities.