Genetic polymorphism analysis of 8 short tandem repeat loci on the X chromosome in Luoba population of China.
- Author:
Long-li KANG
1
;
Yu-ming MA
;
Fang GAO
;
Bing YU
;
Sheng-bin LI
Author Information
- Publication Type:Journal Article
- MeSH: China; Chromosomes, Human, X; genetics; Female; Gene Frequency; Genetics, Population; Genotype; Humans; Male; Microsatellite Repeats; genetics; Polymerase Chain Reaction; Polymorphism, Genetic; genetics
- From: Chinese Journal of Medical Genetics 2007;24(4):479-481
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the alleles and genotype frequency of 8 short tandem repeat (STR) loci (DXS6804, DXS8378, DXS7132, DXS7133, DXS6789, DXS101, HPRTB and DXS7423) on the X chromosome in Luoba ethnic group of China.
METHODSThe eight X chromosome STR (X-STR) loci were analyzed by polymerase chain reaction (PCR) followed by polyacylamide gel electrophoresis and silver stain.
RESULTSAmong 96 unrelated Luoba individuals, the numbers of alleles in the 8 X-STR loci (DXS7133, DXS6789, DXS6804, DXS8378, DXS101, DXS7424, DXS7132 and HPRTB) were 5, 8, 7, 5, 8, 8, 8 and 5, respectively. The genotype frequencies in females were in accordance with Hardy-Weinberg equilibrium.
CONCLUSIONThe eight X-STR loci are appropriate for individual identification, paternity testing involving a female child and studies on related diseases.
