Association between mRNA level of Pde4d and Alox5ap and hypertensive stroke as well as hypertension in rats.
- Author:
Xiao-wei XU
1
;
Wen-li SHENG
;
Liu-Lin
Author Information
- Publication Type:Journal Article
- MeSH: 5-Lipoxygenase-Activating Proteins; Animals; Carrier Proteins; genetics; Cyclic Nucleotide Phosphodiesterases, Type 3; genetics; Cyclic Nucleotide Phosphodiesterases, Type 4; Gene Expression Regulation; Hypertension; complications; genetics; Membrane Proteins; genetics; RNA, Messenger; genetics; metabolism; Rats; Rats, Inbred SHR; Stroke; complications; genetics
- From: Chinese Journal of Medical Genetics 2007;24(5):491-494
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate whether mRNA levels of Pde4d and Alox5ap were associated with hypertensive stroke and hypertension in stroke-prone renovascular hypertensive rats (RHRSP) which could simulate human being's hypertensive cerebral stroke.
METHODSFive groups were established: normotensive group, gradient hypertensive groups I, II and III(with contractive pressure of 140-159 mmHg, 160-179 mmHg and 180-199 mmHg respectively) and spontaneous stroke group. RNA from leukocytes in peripheral blood of each rat underwent real time PCR after reversed.
RESULTSThe mRNA levels of Pde4d and Alox5ap of spontaneous stroke group were statistically higher than that of the other groups. Expression of Pde4d of hypertensive group I was a bit higher than that of normotensive group and hypertensive groups II and III; as for Alox5ap, there was no statistical difference between normotensive group and all gradient hypertensive groups.
CONCLUSIONAnimal experiments come to conclusions that over-expression of Pde4d and Alox5ap are associated with hypertensive stroke but not with hypertension. Therefore, the two genes confer the risk of hypertensive stroke independent of traditional risk factors. It is speculated that over-expression of Pde4d and Alox5ap can motivate onset of hypertensive cerebral stroke by participating in inflammation of arterial walls.
