Sequence analysis of a novel HLA allele B*5618.
- Author:
Hong-yan ZOU
1
;
Zhen LI
;
Chao-peng SHAO
;
Liang-hong CHENG
;
Shi-zheng JIN
;
Dan ZHOU
;
Wen XIONG
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; China; ethnology; Ethnic Groups; genetics; Exons; Gene Frequency; HLA-B Antigens; genetics; Haplotypes; Heterozygote; Humans; Male; Oligonucleotide Probes; genetics; Polymerase Chain Reaction; Sequence Analysis, DNA
- From: Chinese Journal of Medical Genetics 2007;24(5):510-513
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify HLA novel allele in Chinese Han individual.
METHODSAn unknown HLA-B allele which was similar to HLA-B*5610 was detected by polymerase chain reaction-sequence specific oligonucleotide probes(PCR-SSOP), PCR-sequence specific primer(PCR-SSP) and heterozygous sequence-based typing (SBT) in a Chinese Han individual. Its anomalous patterns suggested the possible presence of new allele. The HLA-B*56 allele was amplified separately by using allele-specific primers and sequencing exons 2-4 in both directions. The differences between the novel B*56 allele and B 5610 were identified.
RESULTSThere were 4nt changes from B*5610 in exon 3, at nt379 where C>G (codon 127 CTG>GTG, 127 Leu>Val); nt412 where A>G (codon 138 AAC>GAC, 138 Asn>Asp), nt419 where T>C and nt420 where A>C (codon 140 TTA>TCC, 140 Leu>Ser). The sequence was submitted to Genbank and the accession number was EF016753.
CONCLUSIONThis allele is a novel HLA-B allele, and has been officially named HLA-B*5618 by the WHO Nomenclature Committee in September 2006.
