Triple-color primed in situ labeling protocol of human metaphase chromosomes.
- Author:
Di-shi LIU
1
,
2
;
Xian-ping DING
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Chromosomes, Human; genetics; Color; Feasibility Studies; Humans; Klinefelter Syndrome; genetics; pathology; Lymphocytes; cytology; metabolism; pathology; Male; Metaphase; genetics; Primed In Situ Labeling; methods; Sensitivity and Specificity
- From: Chinese Journal of Medical Genetics 2007;24(5):514-517
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the feasibility of simultaneous detection for several chromosomes with optimized triple-color primed in situ labelling (PRINS) protocol in cultured peripheral blood lymphocytes.
METHODSPre-test of gonosome detection with dual-color PRINS protocol was performed to explore and optimize the order and condition of PRINS primers. A peripheral blood sample from a Klinefelter's syndrome patient (47, XXY) had also been studied with optimized triple-color PRINS to prove the correspondence between the number of signals and chromosomes.
RESULTSChromosome 18, X and Y had been simultaneously and specifically marked within 3 hours. The frequency of successful labeling reached 90% both in dual-color and triple-color test. Two chromosome X had been correctly showed in lymphocyte sample of Klinerfelter's syndrome.
CONCLUSIONNumerical chromosome anomalies could be rapidly and exactly detected with this non-ddNTP-blocking multicolor PRINS protocol in peripheral blood lymphocytes. The results of in situ labeling are much clearer with inner control.
