Identification of a novel HLA allele B 4446 by sequencing-based typing.
- Author:
Jian-ping LI
1
;
Xu ZHANG
;
Xiao-feng LI
;
Zhe QU
;
Jing-bo GAO
;
Xu-ying HUANG
;
Xian-zhi LIU
Author Information
- Publication Type:Journal Article
- MeSH: Alleles; Base Sequence; HLA-B Antigens; classification; genetics; Humans; Male; Molecular Sequence Data; Sequence Analysis, DNA
- From: Chinese Journal of Medical Genetics 2007;24(5):518-519
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify a novel HLA allele in Chinese population.
METHODSA new HLA-B allele was initially detected by an usual PCR-SSP and PCR-SSOP in routine typing HLA allele. Sequence-based typing (SBT) was used to identify and analysis the difference between the new allele and HLA-B 4409 allele.
RESULTSThe HLA-B exon 3 nucleotide sequence of the novel allele was different from all other known alleles. The allele had 3 nucleotides replaced of the closest matching B 4409 allele at nt538(G>C), nt539(A>T) and nt540 (C>G) in exon 3, resulting in an amino acid change from D(GAC) to L(CTG) at codon 180.
CONCLUSIONA novel HLA allele was confirmed by the SBT and it was officially designated as HLA-B 4446 by WHO Nomenclature Committee for Factors of the HLA System in September,2005.
