Comparative study on clinical features between TEL-AML1 positive and negative childhood acute lymphoblastic leukemia.
- Author:
Xia GUO
1
;
Qiang LI
;
Yi-ping ZHU
;
Chen-yan ZHOU
;
Ju GAO
;
Xi-hong LI
;
Ling-li PAN
;
Feng-yi LI
;
Xin TIAN
;
Hai-ting LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Bone Marrow; pathology; Child; Child, Preschool; Core Binding Factor Alpha 2 Subunit; genetics; Female; Gene Fusion; Humans; Infant; Infant, Newborn; Male; Phenotype; Platelet Count; Precursor Cell Lymphoblastic Leukemia-Lymphoma; blood; genetics; immunology; pathology; Prednisone; therapeutic use; Proto-Oncogene Proteins c-ets; genetics; RNA; isolation & purification; Repressor Proteins; genetics
- From: Chinese Journal of Medical Genetics 2007;24(5):560-563
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the incidence of TEL-AML1 fusion gene in childhood acute lymphoblastic leukemia (ALL) and to compare the clinical features between TEL-AML1 positive and negative patients.
METHODSSamples of bone marrow or peripheral blood were collected from 95 newly diagnosed ALL children and the TEL-AML1 fusion gene was detected using nested reverse transcription-polymerase chain reaction (RT-PCR). The ALL patients were stratified into TEL-AML1 positive and negative groups and the clinical features were compared.
RESULTSAmong 95 patients, 20 (21.05%) were TEL-AML1 positive. The median age of TEL-AML1 positive patients was 5.9 years old and M/F ratio was 1.22:1. There were significant differences between TEL-AML1 positive and negative patients in hepatomegaly (2.75 cm vs. 4 cm below costal arch, P=0.006), splenomegaly (0 cm vs. 3 cm below costal arch, P < 0.001), initial white blood cell count (median 7.40 x 10(9)/L vs.18.70 x 10(9)/L, P=0.011), initial peripheral blood blast (median 2.45 x 10(9)/L vs.11.66 x 10(9)/L, P=0.013), hemoglobin level [(61.45 +/- 13.46) g/L vs. (75.89 +/- 23.11) g/L, P=0.003] and serum lactate dehydrogenase [(621.47 +/- 335.85) U/L vs.(1566.64 +/- 1720.45) U/L, P=0.020], while no differences were found between two groups in age, gender ratio, initial platelet count, percentage of blast in bone marrow, immunophenotypes and the expression of myeloid antigen CD13, CD33 and CD34. The prednisone sensitivity test showed that all 12 TEL-AML1 positive patients were good responders, while there were 11 prednisone poor responders among 40 negative patients (27.50%, P < 0.05). Bone marrow examination on day 15 showed no difference in the rate of complete remission between TEL-AML1 positive and negative patients.
CONCLUSIONThe incidence of TEL-AML1 fusion gene in cases of ALL is 21.05%. The load of leukemia cells in TEL-AML1 positive patients is significantly smaller than its counterparts, and the blast cells in TEL-AML1 positive patients are more sensitive to prednisone, indicating childhood ALL with TEL-AML1 fusion gene has a favorable prognosis.
