Screening for the microdeletions of azoospermia factor on the Y chromosome in male infertile patients from Guangzhou.
- Author:
Ya-feng LIU
1
;
Jian-ping OU
;
Can-quan ZHOU
;
Qiong WANG
;
Zi-lian WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Asian Continental Ancestry Group; genetics; Azoospermia; diagnosis; genetics; Case-Control Studies; China; Chromosome Deletion; Chromosomes, Human, Y; genetics; Female; Genetic Loci; Genetic Testing; Humans; Infertility, Male; diagnosis; genetics; Male; Middle Aged; Oligospermia; diagnosis; genetics; Seminal Plasma Proteins; genetics
- From: Chinese Journal of Medical Genetics 2007;24(5):564-566
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship between microdeletion of azoospermia factor (AZF) and male infertility.
METHODSMultiplex PCR was used to detect Y chromosome microdeletion in AZFa, AZFb and AZFc in 103 cases of idiopathic azoospermia, 72 cases of severe idiopathic oligozoospermia, and 60 healthy male controls.
RESULTSNo microdeletion was found in 60 controls. Y chromosome microdeletion was found in 19 of 175 azoospermia patients, the total prevalence rate of microdeletion was 10.9%. There were 15 cases (11 for azoospermia, 4 for severe oligozoospermia) in AZFc (8.6%), 3 cases (1 for azoospermia, 2 for severe oligozoospermia) in AZFb+c (1.7%), 1 case (azoospermia) in AZFa+b+c (0.6%). According to statistics, the difference of microdeletion rate between two groups was significant(P < 0.01).
CONCLUSIONY chromosome microdeletions is an important reason of azoospermia. Screening of Y chromosome microdeletions for azoospermia patients before intracytoplasmic sperm injection treatment is essential.
