Establishment of NCAM L1 minigene model and its splicing patterns in different cell lines.
- Author:
Wei ZHANG
1
;
Quan SHEN
;
Zheng-yu PENG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Genetic Vectors; Humans; Neural Cell Adhesion Molecule L1; genetics; Plasmids; genetics; RNA Splicing; Transfection
- From: Journal of Zhejiang University. Medical sciences 2011;40(4):427-431
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a minigene model of neural cell adhesion molecule L1 (NCAM L1) gene and to study its splicing patterns in different cell lines.
METHODSUsing human genetic cDNA as template, the NCAM L1 minigene fragment was amplified and inserted into eukaryotic expression vector. The minigene was transfected into 4 cell lines and the splicing patterns of NCAM L1 minigene in these cell lines were studied.
RESULTSThe splicing patterns of NCAM L1 minigene were different in individual cell lines. In PFSK and Hela cell lines, two splicied isoforms were generated but in COS-1 and R28 cell lines, only one isoform existed.
CONCLUSIONNCAM L1 minigene model can be used in alternative splicing analysis.
