A new method for simultaneous assay of six organic acids in Lonicerae Japonicae Flos.
- Author:
Wen-long LI
1
;
Wen-ming ZHANG
;
Dong-sheng XUE
;
Jun CHEN
;
Hai-bin QU
Author Information
- Publication Type:Journal Article
- MeSH: Caffeic Acids; analysis; Chlorogenic Acid; analysis; Chromatography, High Pressure Liquid; methods; Lonicera; chemistry; Quality Control; Quinic Acid; analysis; Reproducibility of Results
- From: Journal of Zhejiang University. Medical sciences 2012;41(1):13-18
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method of quantitative analysis of multi-components by single marker(QAMS) for quality control of Lonicerae Japonicae Flos.
METHODSThe contents of chlorogenic acid(CA) and caffeic acid(CfA) were determined, and the relative correction factors(RCF) of other organic acids were calculated, which were used for the indirect determination.
RESULTSThe RCFs for the neochlorogenic acid(NCA), 3, 4-O-dicaffeoylquinic acid(3, 4-DCA), 3, 5-O-dicaffeoylquinic acid(3, 5-DCA), and 4, 5-O-dicaffeoylquinic acid(4, 5-DCA) were 5.462, 5.689, 2.313, 2.382(to CA) and 3.941, 4.103, 1.669, 1.718(to CfA), respectively. The established method was validated in different laboratories with different high performance liquid chromatography(HPLC) instruments and different chromatographic columns; the result indicated that the reproducibility was satisfactory. There was no significant difference between the established QAMS method and external reference method (P>0.05).
CONCLUSIONThe established QAMS method can be used for simultaneous determination of 6 organic acids as quality control of Lonicerae Japonicae Flos with easy available standard substances.
