Screening and identifying hepatotoxic components in Aucklandiae Radix with GC-MS.
- Author:
Xiao-ping ZHAO
1
;
Lin LU
;
Bin HU
;
Shu-fang WANG
Author Information
- Publication Type:Journal Article
- MeSH: Drugs, Chinese Herbal; chemistry; Gas Chromatography-Mass Spectrometry; methods; Lactones; analysis; toxicity; Sesquiterpenes; analysis; toxicity
- From: Journal of Zhejiang University. Medical sciences 2012;41(1):43-46
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen the potential hepatotoxic components in Chinese herb medicine Aucklandiae Radix.
METHODSThe potential hepatotoxic components were screened using HepG2 cells labeled with fluorescein diacetate from 25 fractions of Aucklandiae Radix, in which the hepatotoxic compounds were further identified with GC-MS.
RESULTSTen potential hepatotoxic fractions were screened. The identification results by GC-MS indicated that the main compounds in C09 were dehydrocostuslactone, santamarine (or magnolialide) and reynosin, and in C11 were α-costol and elemol.
CONCLUSIONDehydrocostuslactone, santamarine (or magnolialide), reynosin, α-costol and elemol are potential hepatotoxic compounds in Aucklandiae Radix.
