Cysteinyl leukotriene receptor 1 is involved in rotenone-induced injury of PC12 cells.
- Author:
Shu-ying YU
1
;
Bing ZHAO
;
Xia-yan ZHANG
;
Xaio-yan ZHANG
;
Yan-fang WANG
;
Li-hui ZHANG
;
Yun-bi LU
;
Er-qing WEI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; PC12 Cells; Rats; Receptors, Leukotriene; metabolism; physiology; Rotenone; toxicity
- From: Journal of Zhejiang University. Medical sciences 2012;41(2):139-145
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate whether cysteinyl leukotriene receptor 1 (CysLT₁ receptor) is involved in rotenone-induced injury of PC12 cells.
METHODSAfter 24 h treatment with rotenone or with rotenone and the CysLT₁ receptor antagonist montelukast, PC12 cell viability was determined by the colorimetric MTT reduction assay. After PC12 cells were treated with various concentrations of rotenone for 24 h or with 3 μmol/L rotenone for various durations, the expression of CysLT(1) receptor was determined by Western blotting, and its intracellular distribution was detected by immunocytochemistry.
RESULTSRotenone (0.3-30 μmol/L) induced PC12 cell injury; this injury was significantly attenuated by montelukast at 1 and 5 μmol/L.The expression of CysLT(1) receptor increased after rotenone treatment at 1-10 μmol/L, or at 3 μmol/L for 3 and 24 h. Rotenone caused concentration-and time-dependent translocation of CysLT₁ receptor from the nucleus to the cytosol.
CONCLUSIONCysteinyl leukotriene receptor 1 is involved in rotenone-induced injury of PC12 cells.
