GAD67-GFP expression and co-localization with bNOS in main olfactory bulb of GAD67-GFP knock-in mouse.
- Author:
Fei HAN
1
;
Jing YANG
;
Wen-fu YU
;
Yi-fei YIN
;
Sheng-xi WU
;
Shu-cai LING
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Gene Knock-In Techniques; Glutamate Decarboxylase; genetics; metabolism; Green Fluorescent Proteins; genetics; metabolism; Mice; Mice, Transgenic; Neurons; metabolism; Nitric Oxide Synthase Type I; metabolism; Olfactory Bulb; metabolism; Tissue Distribution
- From: Journal of Zhejiang University. Medical sciences 2012;41(2):159-170
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the distribution of GAD67 and the co-localization with bNOS in the main olfactory bulb of GAD67-GFP knock-in mouse.
METHODSPolymerase chain reaction was applied to identify the genotype of GAD67-GFP knock-in mouse, the animals were sacrificed and frozen sections of olfactory bulb were prepared. The Nissl-staining was performed to show an framework of the neuron in the olfactory bulb. The distribution of GAD67 and co-localization with bNOS were detected by immunofluorescence technique.
RESULTSThe proportion of GAD67-positive cells among DAPI-positive cells were (42.98 ± 0.92)% in glomerular layer, (23.64 ± 0.84)% in mitral cell layer and (77.75 ± 0.84)% in granule cell layer; the bNOS-positive cells mainly existed in glomerular layer and mitral cell layer, very few in granule cell layer. No co-localization of GAD67 and bNOS in granule cell layer and mitral cell layer was found, but there was dispersed distribution in glomerular layer.
CONCLUSIONGAD67-positive neurons mainly appear in glomerular layer and granule cell layer, and the bNOS is mostly expressed in glomerular layer and mitral cell layer; while the co-localization of GAD67 and bNOS only occurs in glomerular layer of olfactory bulb.
