Evaluation of the protein requirement in Chinese young adults using the indicator amino acid oxidation technique.
- Author:
Min LI
1
;
Zhi Ling WANG
;
Ling Yan GOU
;
Wei Dong LI
;
Yuan TIAN
;
Yi Chun HU
;
Rui WANG
;
Jian Hua PIAO
;
Xiao Guang YANG
;
Yu Hui ZHANG
Author Information
- Publication Type:Journal Article
- Keywords: Adult protein intake; Biphasic linear regression crossover analysis; Indicator amino acid oxidation; Mixed diet; Protein requirement
- MeSH: Adult; Amino Acids; metabolism; Body Composition; Body Weight; Breath Tests; Carbon Dioxide; analysis; Dietary Proteins; administration & dosage; Female; Humans; Male; Nutritional Requirements; Oxidation-Reduction; Young Adult
- From: Biomedical and Environmental Sciences 2013;26(8):655-662
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo accurately calculate the protein requirements in Chinese young adults using the indicator amino acid oxidation technique.
METHODSNine women and ten men received a restricted daily level of protein intake (0.75, 0.82, 0.89, 0.97, and 1.05 g/kg), along with L-[1-13C]-leucine. Subjects' protein requirement was determined by a biphasic linear regression crossover analysis of F13CO2 data. In doing so, a breakpoint at the minimal rate of appearance of 13CO2 expiration specific to each level of dietary protein was identified. This trial was registered with the Chinese clinical trial registry as ChiCTR-ONC-11001407.
RESULTSThe Estimated Average Requirement (EAR) and the Recommended Nutrient Intake (RNI) of protein for healthy Chinese young adults were determined to be 0.87 and 0.98 g/(kg•d), respectively, based on the indicator amino acid oxidation technique.
CONCLUSIONThe EAR and RNI of mixed protein are 5% and 16% that are lower than the current proposed EAR and RNI (0.92 and 1.16 g/(kg•d), respectively), as determined by the nitrogen balance method. The respective EAR and RNI recommendations of 0.87 and 0.98 g/(kg•d) of mixed protein are estimated to be reasonable and suitable for Chinese young adults.
