N-hexane alters the maturation of oocytes and induces apoptosis in mice.
- Author:
Jin LIU
1
;
Lei HUANG
;
Yan SUN
;
Yu Chen LI
;
Jian Lin ZHU
;
Wen Xiang WANG
;
Wen Chang ZHANG
Author Information
- Publication Type:Journal Article
- Keywords: Fertilization; Maturation; Mitochondrial membrane potential; N-hexane
- MeSH: Animals; Apoptosis; drug effects; Environmental Pollutants; toxicity; Female; Fertilization; drug effects; Hexanes; toxicity; Male; Membrane Potential, Mitochondrial; drug effects; Mice; Mice, Inbred ICR; Oocytes; drug effects; growth & development
- From: Biomedical and Environmental Sciences 2013;26(9):735-741
- CountryChina
- Language:English
-
Abstract:
OBJECTIVEThis study was aimed to determine the effects of n-hexane on the maturation of mouse oocytes.
METHODSCell culture was used to observe the maturation of mouse oocytes and CLSM was employed to determine their apoptosis.
RESULTSGerminal vesicle breakdown (GVBD) and extrusion of the first polar body in mouse oocytes were significantly inhibited by n-hexane. After fertilization, the number of eggs in the mouse was significantly reduced by n-hexane. Mitochondrial membrane potentials (ΔΨm) were altered in mouse oocytes that were leading to apoptosis of the oocytes.
CONCLUSIONN-hexane might have affected the maturation of oocytes, causing alteration of ΔΨm and leading to apoptosis which maybe one of the most important mechanisms.
