Protein expression in silica dust-induced transdifferentiated rats lung fibroblasts.
- Author:
Chang Fu HAO
1
;
Xiao Fang LI
;
Wu YAO
Author Information
- Publication Type:Journal Article
- Keywords: Crystalline silica; Myofibroblast; Transdifferentiation; Two-dimensional electrophoresis
- MeSH: Animals; Cell Transdifferentiation; Electrophoresis, Gel, Two-Dimensional; Fibroblasts; metabolism; Macrophages, Alveolar; physiology; Male; Rats; Silicon Dioxide; Silicosis; etiology
- From: Biomedical and Environmental Sciences 2013;26(9):750-758
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo analyze the expression of different proteins in free silica-induced transdifferentiated rat lung fibroblasts.
METHODSRat lung fibroblasts and alveolar macrophages were cultured. A transdifferentiation model of rat lung fibroblasts was established. Free silica was used as a stimulator for rat lung fibroblasts. Changes in α-SMA were detected by immunohistochemistry and Western blot, respectively. Protein of lung fibroblasts was extracted and analyzed by two-dimensional electrophoresis (2-DE).
RESULTSSix protein spots were identified by mass spectrometry, including glyceraldehyde 3-phosphate-dehydrogenase, peroxiredoxin 5, heterogeneous nuclear ribonucleoprotein A2, transgelin 2, keratin K6 and vimentin.
CONCLUSIONSome proteins are changed in free silica-induced transdifferentiated rat lung fibroblasts.
