All-transretinoic acid regulates Th1/Th2 balance in CD4+ T cells when GATA-3 is deficient.
10.3967/0895-3988.2013.09.010
- Author:
Yan Feng ZHU
1
;
Jia Zhe HU
;
Pin Nan ZHAO
;
Lin Xi LIU
;
Yun LI
Author Information
1. Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan University, Chengdu 610041, Sichuan, China.
- Publication Type:Letter
- MeSH:
CD4-Positive T-Lymphocytes;
drug effects;
Cell Differentiation;
drug effects;
Cells, Cultured;
GATA3 Transcription Factor;
deficiency;
Humans;
Th1-Th2 Balance;
drug effects;
Tretinoin;
pharmacology
- From:
Biomedical and Environmental Sciences
2013;26(9):774-777
- CountryChina
- Language:English
-
Abstract:
The essential effect of vitamin A on immune function occurs through various mechanisms including direct effect on Th1-Th2 balance modulation. However, it is unclear whether or not vitamin A can regulate Th1-Th2 balance under a strong Th1-polarizing condition. Therefore, the purpose of our study was to examine the effect of vitamin A metabolite all-trans retinoic acid (ATRA) on Th1-Th2 differentiation in CD4+ T cells under GATA-3 deficiency, which can induce Th1-polarizing condition. In the present study, GATA-3 deficiency T cells were induced by siRNA and checked by real-time quantitative PCR and western blot. GATA-3 deficiency CD4+ T cells and normal CD4+ T were treated for 48 h with or without ATRA. The expression of Th1 and Th2 cytokines were detected by qPCR and ELISA. The results would contribute to clarify the knowledge of the role of vitamin A in regulating Th1-Th2 balance under some special conditions, and help to explain the mechanism of immune regulatory function of vitamin A.
