Molecular epidemiological characteristics of Streptococcus pyogenes strains involved in an outbreak of scarlet fever in China, 2011.
- Author:
Yuan Hai YOU
1
;
Yan Yan SONG
;
Xiao Mei YAN
;
Hai Bin WANG
;
Meng Han ZHANG
;
Xiao Xia TAO
;
Lei Lei LI
;
Yu Xin ZHANG
;
Xi Hong JIANG
;
Bing Hua ZHANG
;
Hao ZHOU
;
Di XIAO
;
Lian Mei JIN
;
Zi Jian FENG
;
Feng Ji LUO
;
Jian Zhong ZHANG
Author Information
- Publication Type:Journal Article
- Keywords: Molecular epidemiology; Scarlet fever; Streptococcus pyogenes
- MeSH: Anti-Bacterial Agents; administration & dosage; therapeutic use; Child; China; epidemiology; Disease Outbreaks; Drug Resistance, Bacterial; Electrophoresis, Gel, Pulsed-Field; Humans; Incidence; Molecular Epidemiology; Scarlet Fever; drug therapy; epidemiology; microbiology; Streptococcus pyogenes; drug effects; genetics; isolation & purification; pathogenicity; Virulence
- From: Biomedical and Environmental Sciences 2013;26(11):877-885
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate molecular characterization of streptococcus pyogenes isolates involved in an outbreak of scarlet fever in China in 2011.
METHODSSeventy-four Streptococcal pyogenes involved in an outbreak of scarlet fever were isolated from pediatric patients in the areas with high incidence in China from May to August of 2011. Emm genotyping, pulsed-field gel electrophoresis (PFGE), superantigen (SAg) genes and antimicrobial susceptibility profiling were analyzed for these isolates.
RESULTSA total of 4 different emm types were identified. Emm12 was the most prevalent type which contained four predominating PFGE patterns corresponding to four different virulence and superantigen profiles. Emm12 (79.7%) and emm1 (14.9%) accounted for approximately 94% of all the isolates. The speA gene was all negative in emm12 isolates and positive in emm1 isolates. All strains were resistant to erythromycin, and 89.4% of them were resistant to erythromycin, tracycline, and clindamycin simultaneously.
CONCLUSIONSeveral highly diversified clones with a high macrolide resistance rate comprise a predominant proportion of circulating strains, though no new emm type was found in this outbreak. The data provide a baseline for further surveillance of scarlet fever, which may contribute to the explanation of the outbreak and development of a GAS vaccine in China.
