Role of insulin-like growth factor II receptor in transdifferentiation of free silica-induced primary rat lung fibroblasts.
- Author:
Chang Fu HAO
1
;
Xiao Fang LI
;
Wu YAO
Author Information
- Publication Type:Journal Article
- Keywords: Insulin-like growth factor II receptor; Lung fibroblasts; Silicosis; Transdifferentiation
- MeSH: Animals; Base Sequence; Cell Differentiation; physiology; Cells, Cultured; DNA Primers; Fibroblasts; drug effects; Lung; cytology; drug effects; Male; RNA, Messenger; genetics; Rats; Rats, Sprague-Dawley; Receptor, IGF Type 2; genetics; physiology; Silicon Dioxide; pharmacology
- From: Biomedical and Environmental Sciences 2013;26(12):979-985
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the role of insulin-like growth factor II receptor in free silica-induced transdifferentiation of primary rat lung fibroblasts.
METHODSRat lung fibroblasts and rat alveolar macrophages were cultured. A transdifferentiation model of primary rat lung fibroblasts was induced by free silica. Levels of α-SMA protein, IGF-IIR protein and mRNA were measured by immunocytochemistry, Western blot and RT-PCR, respectively. Lung fibroblasts were treated with Wortmannin.
RESULTSThe expression levels of α-SMA and IGF-IIR increased with the increasing free silica concentration and decreased after Wortmannin was used.
CONCLUSIONThe IGF-IIR plays an important role in free silica-induced transdifferentiation of primary rat lung fibroblasts.
