Mitochondria DNA 4977 bp common deletion in peripheral whole blood from healthy donors.

Author: Ping WANG ¹ ; Yu Long LIU ; Lin HAN ; Feng Ling ZHAO ; Fei GUO ; Xi Ai WANG ; Yu Min LV
Author Information

1. Henan Institute of Occupational Medicine, Zhengzhou 450052, Henan, China.
Publication Type:Journal Article
MeSH: Base Sequence; DNA Primers; DNA, Mitochondrial; genetics; Female; Humans; Male; Real-Time Polymerase Chain Reaction; Sequence Deletion
From: Biomedical and Environmental Sciences 2013;26(12):990-993
CountryChina
Language:English
Abstract: To investigate the distribution of mitochondria DNA 4 977 bp deletion, a common deletion (CD), in normal populations of Chinese, human peripheral blood samples from sixty healthy donors were collected, and levels of the CD in genomic DNA from the samples were detected using real-time PCR. The results showed that the CD was found in 27 health donors, with its positive rate being 45% (27/60). The CD ratio was between 0 and 0.00308%, and not affected by age and gender in sixty healthy donors. Our studies indicate that the CD ratio is low, and do not show the age-dependent accumulation and any gender difference in peripheral whole blood from the normal Chinese population.