Partial Nucleotide Sequence of Porcine Parvovirus (VRI-1 Strain): Identification of the Putative Defective Genomes.
- Author:
Byoung Han KIM
1
;
Soo Hwan AN
;
Chang Hee KWEON
;
Jae Young SONG
;
Jong Hyeon PARK
;
Bang Hun HYUN
;
Dong Kun YANG
;
Ryun Bin TAK
Author Information
1. National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry, Anyang 430-824, Korea. kimbh@nvrqs.go.kr
- Publication Type:Original Article
- Keywords:
Porcine parvovirus;
VRI-1 strain;
Nucleotide sequence
- MeSH:
Base Sequence*;
Clone Cells;
DNA;
DNA, Viral;
Genome*;
Genome, Viral;
Nucleotides;
Parvovirus, Porcine*
- From:Journal of Bacteriology and Virology
2004;34(3):201-212
- CountryRepublic of Korea
- Language:English
-
Abstract:
A porcine parvovirus, designated as VRI-1, was isolated from a 30-day-old piglet. Replicative form of viral DNA from ST cells infected with VRI-1 was directly cloned into pUC19. The cloned DNA fragment contained the entire nonstructural and structural protein genes, covering approximately 85% of the viral genome. The nucleotide sequence of VRI-1 showed 99.4~99.5% identity in the nonstructural protein (NS) and 99.0~99.2% identity in the structural protein with previously reported PPV strains, respectively. Among the cloned genes, two types of defective genomes with deletion of 100 and 247 nucleotides at almost similar location of 3' region within NS gene were also identified in this study.
