The development of a fluorescence polarization immunoassay for aflatoxin detection.

Author: Ya Jie SHENG ¹ ; Sergei EREMIN ² ; Tie Jun MI ¹ ; Su Xia ZHANG ¹ ; Jian Zhong SHEN ¹ ; Zhan Hui WANG ¹
Author Information

1. Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, and Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, Beijing 100193, China.
2. Department of Chemistry, M.V. Lomonosov Moscow State University, Moscow 109807, Russia.
Publication Type:Letter
MeSH: Aflatoxins; analysis; Fluorescence Polarization Immunoassay
From: Biomedical and Environmental Sciences 2014;27(2):126-129
CountryChina
Language:English
Abstract: A fluorescence polarization immunoassay (FPIA) was developed for the analysis ofaflatoxins (AFs) using an anti-aflatoxin B1 (AFB1) monoclonal antibody and a novel fluorescein-labeled AFB1 tracer. The FPIA showed an IC50 value of 23.33 ng/mL with a limit of detection of 13.12 ng/mL for AFB1. The cross-reactivities of AFB1, AFB2, AFG1, AFG2, AFM1, and AFM2 with the antibody were 100%, 65.7%, 143%, 23.5%, 111.4%, and 2%, respectively. The group-specificity of anti-AFB1mAb indicated that the FPIA could potentially be used in a screening method for the detection of total AFs, albeit not AFG2 and AFM2. The total time required for analyzing 96 samples in one microplate was less than 5 min. This study demonstrates the potential usefulness of the FPIA as a rapid and simple technique for monitoring AFs.