Lateral flow immunoassay for quantitative detection of ractopamine in swine urine.

Author: Mei Ling REN ¹ ; Xue Lan CHEN ² ; Chao Hui LI ¹ ; Bo XU ³ ; Wen Juan LIU ³ ; Heng Yi XU ¹ ; Yong Hua XIONG ¹
Author Information

1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, Jiangxi, China.
2. Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Jiangxi Normal University, Nanchang 330022, Jiangxi, China.
3. Wuxi Zodoboer Biotech. Co., Ltd., Wuxi 214174, Jiangsu, China.
Publication Type:Letter
MeSH: Animals; Immunoassay; Phenethylamines; urine; Reagent Strips; Swine; urine
From: Biomedical and Environmental Sciences 2014;27(2):134-137
CountryChina
Language:English
Abstract: A strip reader based lateral flow immunoassay (LFIA) was established for the rapid and quantitative detection of ractopamine (RAC) in swine urine. The ratio of the optical densities (ODs) of the test line (AT) to that of the control line (AC) was used to effectively minimize interference among strips and sample variations. The linear range for the quantitative detection of RAC was 0.2 ng/mL to 3.5 ng/mL with a median inhibitory concentration (IC50) of 0.59 ± 0.06 ng/mL. The limit of detection (LOD) of the LFIA was 0.13 ng/mL. The intra-assay recovery rates were 92.97%, 97.25%, and 107.41%, whereas the inter-assay rates were 80.07%, 108.17%, and 93.7%, respectively.