Different expression of TGF-beta1 and collagen during the healing process of wound infected by Pseudomonas aeruginosa.
- Author:
Lian-bo ZHANG
1
;
Jian-ning LI
;
Shan-shan LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Collagen Type I; metabolism; Collagen Type III; metabolism; Female; Pseudomonas Infections; pathology; Pseudomonas aeruginosa; Rats; Rats, Wistar; Skin Transplantation; Surgical Wound Infection; metabolism; microbiology; Transforming Growth Factor beta1; metabolism; Wound Healing
- From: Chinese Journal of Plastic Surgery 2011;27(4):272-276
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the different expression of TGF-beta1 and collagen during the healing process of wound infected by Pseudomonas aeruginosa (PAO1).
METHODS24 female Wistar rats were randomly divided into pure wound group (group A) and wound + PAO1 group (group B). The re-epithelial rate, shrinkage rate and neutrophils number on the wounds were observed on the 1st, 3rd, 7th and 10th day after operation. The expression of TGF-beta1 and collage I, Ill was also detected.
RESULTSOn the 7th day, the re-epithelial rate in group A was higher than that in group B, while the shrinkage rate in group A was lower than that in group B. The neutrophils number increased to peak on the 1st day in group B, but on the 3rd day in group A. The TGF-beta1 expression increased after operation in both groups, but it decreased in group B on the 3rd day and re-increased after that. The TGF-beta1 expression was significantly different between the two groups on the 7th day (P < 0.05). The expression of collagen I and III decreased during healing. The expression of collagen III in group A was higher on the 3rd day and was lower on the 7th and 10th day than that in group B, showing a significant difference (P < 0.05).
CONCLUSIONSPAO1 infection could delay the expression of TGF-beta1 and collagen I, III on wound, which may interfere the healing process of wound.
