Experimental study on the induced differentiation of human amnion mesenchymal cells into osteoblasts.
- Author:
Jun SHU
1
;
Li-Li GUO
;
Ke-Hua ZHANG
;
Zhe CAI
;
Li-Ming CHENG
;
Rong-Qi LI
;
Ying CHEN
;
Lin PAN
;
Hua OU
;
Xiao-Jie HUANG
;
Xiang-Li ZHANG
;
Yang XU
;
Yan GAO
;
Xiao-Hui CUI
;
Hong-Xia ZHANG
;
Jia-Xin LIU
Author Information
- Publication Type:Journal Article
- MeSH: Amnion; cytology; Cell Differentiation; Cells, Cultured; Humans; Mesenchymal Stromal Cells; cytology; Osteoblasts; cytology; Osteogenesis; Tissue Engineering; methods
- From: Chinese Journal of Plastic Surgery 2011;27(5):362-367
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the feasibility of inducing differentiation of the human amniotic mesenchymal cells (hAMCs) into osteoblasts in vitro, so as to provide the seed cells for bone tissue engineering.
METHODSThe hAMCs were isolated from abandoned human amnion and cultured in osteogenic media to induce the osteogenic differentiation in vitro. After hAMCs were induced by osteogenic media for 15 days, morphological observation, immunocytochemistry and western blot were used to study the cellular morphology and expression of alkaline phosphatase (ALP), type I collagen, osteopontin and osteocalcin.
RESULTSThe primary cultured hAMCs had long spindle shape or irregular shape, which were distributed evenly. The cells were usually suheultured in 5 or 7 days. After subculture, the cells became larger. After cultured by osteogenic media for 15 days, the hAMCs were detected to express ALP, osteocalcin and osteopontin, and secrete type I collagen.
CONCLUSIONSThe hAMCs are isolated, cultured and amplified easily in vitro. The induced differentiated cells by osteogenic media have typical osteoblast morphological and functional characteristics, which can be used as seed cells for bone tissue engineering.
